背景：ASAH2 （N-acylsphingosine amidohydrolase 2）, also known as neutral ceramidase, is a type II integral membrane protein that can be cleaved to produce a soluble secreted protein.
The enzyme is abundant in the brush border membranes of the intestine, and also expressed in several tissues such as kidney, brain and liver.
The primary structure of ASAH2/neutral ceramidase is highly conserved from bacteria to humans, however, there is a clear difference in the molecular architecture.
The murine ASAH2 possesses ＆lsquo;amucin box＆rsquo;, a Ser/Thr/Pro-rich domain glycosylated with O-glycans which is necessary to retain the enzyme on the plasma membrane as a type II integral protein.
The major physiological function of ASAH2/neutral ceramidase is the metabolism of dietary sphingolipids, and thus plays a role in the generation of messenger molecules such as sphingosine and sphingosine 1-phosphate.
仕様
サイズ：50ug
種由来：Mouse
発現宿主：HEK293 Cells
純度：＞97 ％ as determined by reducing SDS-PAGE.
エンドトキシン濃度：＜1.0 EU per μg of the protein as determined by the LAL method.
アクセッションNo.：NP_061300.1
アミノ酸配列：Thr 34-Thr 756
遺伝子構造：A DNA sequence encoding the Lumenal domain （Thr 34-Thr 756） of mouse ASAH2 （NP_061300.1） precursor was expressed with a N-terminal polyhistidine tag.
別名（遺伝子名）：AI585898
分子量：82 kDa
組成：Lyophilized from sterile PBS, pH 7.4
保存条件：Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at ＜ -20℃ for 3 months.
