背景：Carbonic anhydrases （CAs） are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide.
CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid.
CA12, also known as Car12 and carbonic anhydrase XII, is a type I membrane enzyme of an N-terminal extracellular catalytic domain, a membrane-spanning α-helix, and a small intracellular C-terminal domain.
It is highly expressed in colon, kidney, prostate, intestine and activated lymphocytes and moderately expressed in pancreas, ovary, and testis.
Overexpression of the CA12 is observed in certain human cancers and is used as a tumor marker.
rmCA12 corresponds to the extracellular domain and has both carbonic anhydrase activity and esterase activity.
仕様
サイズ：50ug
種由来：Mouse
発現宿主：HEK293 Cells
純度：＞95 ％ as determined by reducing SDS-PAGE.
エンドトキシン濃度：＜1.0 EU per μg of the protein as determined by the LAL method.
アクセッションNo.：NP_848483.2
アミノ酸配列：Met 1-Ser 301
遺伝子構造：A DNA sequence encoding the extracellular domain （Met 1-Ser 301） of mouse CA12 （NP_848483.2） precursor was expressed with a C-terminal polyhistidine tag.
保存条件：Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at ＜ -20℃ for 3 months.
