背景：Mast Cell Protease 1 （MMCP-1）, also known as MCP-1, MCPT-1 and ＆beta;-chymase, is a member of the Chymase family of chymotrypsin-like serine proteases.
MCPT-1 is a 26 kDa ＆beta;-chymase that is a component of mast cell granules.
It is a 226 amino acid （aa） protein that has a conserved pattern of six cysteines and one potential glycosylation site.
The granule-derived mouse mast cell proteases-1 and -2 （mMCP-1 and -2） colocalize in similar quantities in mucosal mast cells but micrograms of mMCP-1 compared with nanograms of mMCP-2 are detected in peripheral blood during intestinal nematode infection.
mMCP-1 isolated from serum is complexed with serpins and both the accumulation and the longevity of mMCP-1 in blood is due to complex formation, protecting it from a pathway that rapidly clears mMCP-2, which is unable to form complexes with serpins.
The mucosal mast cell （MMC） granule-specific beta-chymase, mouse mast cell protease-1 （mMCP-1）, is released systemically into the bloodstream early in nematode infection before parasite-specific IgE responses develop and TGF-beta1 induces constitutive release of mMCP-1 by homologues of MMC in vitro.
Expression of mMCP-1 is largely restricted to intraepithelial MMC and is thought to play a role in the regulation of epithelial permeability.
Its activation is completed by the removal of a two residue N-terminal propeptide by a dipeptidyl peptidase （Cathepsin C）.
MCPT-1 is upregulated in the intestine in response to nematode infection, or in systemic mucosa in response to anaphylaxis.
Like human ＆alpha;-chymase, MCPT-1 is capable of the conversion of angiotensin I to angiotensin II, which plays a key role in the regulation of arterial pressure.
The intestinal inflammation associated with gastrointestinal helminths is partly mediated by mMCP-1.
仕様
サイズ：50ug
種由来：Mouse
発現宿主：HEK293 Cells
純度：＞97 ％ as determined by reducing SDS-PAGE.
エンドトキシン濃度：＜1.0 EU per μg of the protein as determined by the LAL method.
アクセッションNo.：NP_032596.1
アミノ酸配列：Met 1-Lys 246
遺伝子構造：A DNA sequence encoding the mouse MCPT1 （NP_032596.1） （Met 1-Lys 246） was expressed with a polyhistidine tag at the C-terminus.
別名（遺伝子名）：AV080368;Mcp-1
分子量：26.8 kDa
組成：Lyophilized from sterile PBS, pH 7.4
保存条件：Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at ＜ -20℃ for 3 months.
