背景：Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B （PCPB）, is a highly pancreas -specific protein （PASP）, and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection.
As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg.
The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 307-aa mature chain.
The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.
仕様
サイズ：50ug
種由来：Mouse
発現宿主：HEK293 Cells
純度：＞94 ％ as determined by reducing SDS-PAGE.
エンドトキシン濃度：＜1.0 EU per μg of the protein as determined by the LAL method.
アクセッションNo.：NP_083982.1
アミノ酸配列：Met 1-Tyr 415
遺伝子構造：A DNA sequence encoding the mouse CPB1 （NP_083982.1） precursor （Met 1-Tyr 415） was expressed, with a C-terminal polyhistidine tag.
組成：Lyophilized from sterile 20mM MES, 150mM NaCl, pH 6.5
保存条件：Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at ＜ -20℃ for 3 months.
