背景：Ectonucleotide pyrophosphatase / phosphodiesterase family member 7, also known as Alkaline sphingomyelin phosphodiesterase, Intestinal alkaline sphingomyelinase, Alk-Smase, ENPP7 and NPP-7, is a single-pass type I membrane protein which belongs to thenucleotide pyrophosphatase / phosphodiesterase family.
ENPP7 / NPP-7 is expressed in the intestines and human bile.
ENPP7 / NPP-7 is localized at the surface of the microvillar membrane in small intestine enterocytes, as well as in endosome-like structures and in Golgi complex.
The main function of ENPP7 / NPP-7 is to convert the dietary sphingomyelin into ceramide, the sphingolipid messengers via hydrolyzation.
ENPP7 / NPP-7 is also reported to exert a phospholipase C activity toward palmitoyl lyso-phosphocholine.
The activity of this enzyme is inhibited in a dose dependent manner by ATP, imidazole, orthovanadate and zinc ion.
Further, It has been shown in studies that decreased levels of ENPP7 / NPP-7 may be associated with human colon cancer.
仕様
サイズ：50ug
種由来：Mouse
発現宿主：HEK293 Cells
純度：＞90 ％ as determined by reducing SDS-PAGE.
エンドトキシン濃度：＜1.0 EU per μg of the protein as determined by the LAL method.
アクセッションNo.：Q3TIW9
アミノ酸配列：Met 1-Gln 421
遺伝子構造：A DNA sequence encoding the mouse ENPP7 （Q3TIW9） （Met 1-Gln 421） was expressed, with a polyhistidine tag at the C-terminus.
別名（遺伝子名）：Alk-SMase;Gm254
分子量：47 kDa
組成：Lyophilized from sterile 20mM Tris, 150mM NaCl, pH 7.6
保存条件：Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at ＜ -20℃ for 3 months.
