背景：Tie-1/Tie （tyrosine kinase with Ig and EGF homology domains 1） and Tie-2/Tek comprise a receptor tyrosine kinase （RTK） subfamily with unique structural characteristics： two immunoglobulin-like domains flanking three epidermal growth factor （EGF）-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region.
These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis.
Human Tie-2 cDNA encodes a 1124 amino acid （aa） residue precursor protein with an 18 residue putative signal peptide, a 727 residue extracellular domain and a 354 residue cytoplasmic domain.
Two ligands, angiopoietin-1 （Ang-1） and angiopoietin-2 （Ang-2）, which bind Tie-2 with high-affinity have been identified.
Ang-2 has been reported to act as an antagonist for Ang-1.
Mice engineered to overexpress Ang-2 or to lack Ang-1 or Tie-2 display similar angiogenesis defects.
仕様
サイズ：10ug
種由来：Human
発現宿主：HEK293 Cells
純度：＞95 ％ as determined by reducing SDS-PAGE.
エンドトキシン濃度：＜1.0 EU per μg of the protein as determined by the LAL method.
アクセッションNo.：NP_000450.2
アミノ酸配列：Ala23-Lys745
遺伝子構造：Recombinant Human Tie-2 is produced by our Mammalian expression system and the target gene encoding Ala23-Lys745 is expressed with a Fc tag at the C-terminus.
組成：Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
保存条件：Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at ＜ -20℃ for 3 months.
