特徴

• 背景：RCA1,also named as RNF53,plays a central role in DNA repair by facilitating cellular response to DNA repair.
• It is required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle.
• The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair,ubiquitination and transcriptional regulation to maintain genomic stability.
• BRCA1 acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function.
• It is involved in transcriptional regulation of P21 in response to DNA damage.
• BRCA1 is required for FANCD2 targeting to sites of DNA damage.
• It may function as a transcriptional regulator.
• BRCA1 inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation.
• The antibody is specific to BRCA1.
• BRCA1 appears to produce multiple splice variants.
• BRCA1 is a nuclear protein with a molecular mass of 220 kDa.
• The present study describes the isolation and expression of two cDNAs of BRCA1,including a splice variant designated BRCA1D672-4095.
• BRCA1D672-4095 is generated by exclusion of exon 11 by in-frame splicing and produces a 97 kDa protein.
• In contrast to BRCA1,BRCA1D672-4095 localizes to the cytoplasm.