背景：Carboxypeptidase E （CPE）, also known as Carboxypeptidase H, is a peripheral membrane protein and a zinc metallocarboxypeptidase, and the conversion of proCPE into CPE occurs primarily in secretory vesicles.
The active form of CPE cleaves C-terminal amino acid residues of the peptide, and is thus involved in the biosynthesis of peptide hormones and neurotransmitters including insulin, enkephalin, etc.
The enzymatic activity is enhanced by millimolar concentrations of Co2+.
It has also been proposed that membrane-associated carboxypeptidase E acts as a sorting receptor for targeting regulated secretory proteins which are mostly prohormones and neuropeptides in the trans-Golgi network of the pituitary and in secretory granules into the secretory pathway.Its interaction with glycosphingolipid-cholesterol rafts at the TGN facilitates the targeting.
Mutations in this gene are implicated in type II diabetes due to impaired glucose clearance and insulin resistance.
仕様
サイズ：50ug
種由来：Human
発現宿主：HEK293 Cells
純度：＞90 ％ as determined by reducing SDS-PAGE.
エンドトキシン濃度：＜1.0 EU per μg of the protein as determined by the LAL method.
アクセッションNo.：NP_001864.1
アミノ酸配列：Met 1-Ser 453
遺伝子構造：A DNA sequence encoding the human carboxypeptidase E （CPE） precursor （NP_001864.1） （Met 1-Ser 453） was fused with a polyhistidine tag at the C-terminus.
別名（遺伝子名）：Carboxypeptidase E（CPE for short）; Carboxypeptidase H; Enkephalin convertase; Prohormone-processing carboxypeptidase
分子量：49.4 kDa
組成：Lyophilized from sterile PBS, pH 7.4
保存条件：Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at ＜ -20℃ for 3 months.
