背景：Granzyme B, also known as GZMB, is the most prominent member of the granzyme family of cell death-inducing serine proteases expressed in the granules of cytotoxic T lymphocytes （CTLs） and NK cells.
Granzyme B enters the target cells depending on another membrane-binding granule protein, perforin, results in the activation of effector caspases and mitochondrial depolarization through caspase-dependent and -independent pathways, and consequently induces rapid cell apoptosis.
Over 30 substrates of GZMB have been identified including the key substrate caspase-3, ICAD and Bid.
GZMB is suggested to protect the host by lysing cells bearing on their surface 'nonself' antigens such as bacterial and viral infected-cells and tumor cells, and accordingly plays an essential role in immunosurveillance.
仕様
サイズ：50ug
種由来：Human
発現宿主：HEK293 Cells
純度：＞97 ％ as determined by reducing SDS-PAGE.
エンドトキシン濃度：＜1.0 EU per μg of the protein as determined by the LAL method.
アクセッションNo.：NP_004122.1
アミノ酸配列：Met 1-Tyr 247
遺伝子構造：A DNA sequence encoding the proform of human Granzyme B （NP_004122.1） （Met 1-Tyr 247） was expressed with a C-terminal polyhistidine tag.
保存条件：Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at ＜ -20℃ for 3 months.
