背景：Lysosomal carboxypeptidase, cathepsin A （protective protein, CathA）, is a component of the lysosomal multienzyme complex along with beta-galactosidase （GAL） and sialidase Neu1, where it activates Neu1 and protects GAL and Neu1 against the rapid proteolytic degradation.
Cathepsin A is a multicatalytic enzyme with deamidase and esterase in addition to carboxypeptidase activities.
It was recently identified in human platelets as deamidase.
In vitro, it hydrolyzes a variety of bioactive peptide hormones including tachykinins, suggesting that extralysosomal cathepsin A plays a role in regulation of bioactive peptide functions.
It is a member of the alpha/beta hydrolase fold family and has been suggested to share a common ancestral relationship with other alpha/beta hydrolase fold enzymes, such as cholinesterases.
Cathepsin A defects are linked to multiple forms of Galactosialidosis with a combined secondary deficiency of beta-galactosidase and neuraminidase.
Cathepsin A is a key molecule in the onset of galactosialidosis and also highlight the therapeutic acts in vivo as an endothelin-1-inactivating enzyme and strongly confirm a crucial role of this enzyme in effective elastic fiber formation.
仕様
サイズ：50ug
種由来：Human
発現宿主：HEK293 Cells
純度：＞90 ％ as determined by reducing SDS-PAGE.
エンドトキシン濃度：＜1.0 EU per μg of the protein as determined by the LAL method.
アクセッションNo.：NP_001121167.1
アミノ酸配列：Met 1-Tyr 480
遺伝子構造：A DNA sequence encoding the human cathepsin A isoform b （Met 1-Tyr 480） （NP_001121167.1） was expressed with a N-terminal signal peptide and a C-terminal polyhistidine tag.
組成：Lyophilized from sterile 25mM Tris, 0.15mM NaCl, pH 7.5
保存条件：Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at ＜ -20℃ for 3 months.
