背景：phospholipase A2, also known as Phosphatidylcholine 2-acylhydrolase 1B, Group IB phospholipase A2, PLA2 and PLA2G1B, is a secreted protein which belongs to thephospholipase A2 family.
Phospholipase A2 / PLA2G1B catalyzes the release of fatty acids from glycero-3-phosphocholines.
The best known varieties are the digestive enzymes secreted as zymogens by the pancreas of mammals.
Sequences of pancreatic Phospholipase A2 / PLA2G1B enzymes from a variety of mammals have been reported.
One striking feature of these enzymes is their close homology to venom phospholipases of snakes.
Other forms of Phospholipase A2 / PLA2G1B have been isolated from brain, liver, lung, spleen, intestine, macrophages, leukocytes, erythrocytes, inflammatory exudates, chondrocytes, and platelets.
Mice lacking in Phospholipase A2 / PLA2G1B are resistant to obesity and diabetes induced by feeding a diabetogenic high-fat/high-carbohydrate diet.
Oral supplementation of a diabetogenic diet with the PLA2G1B inhibitor methyl indoxam effectively suppresses diet-induced obesity and diabetes.
PLA2G1B inhibition may be a potentially effective oral therapeutic option for treatment of obesity and diabetes.
仕様
サイズ：50ug
種由来：Human
発現宿主：HEK293 Cells
純度：＞92 ％ as determined by reducing SDS-PAGE.
エンドトキシン濃度：＜1.0 EU per μg of the protein as determined by the LAL method.
アクセッションNo.：NP_000919.1
アミノ酸配列：Met 1-Ser 148
遺伝子構造：A DNA sequence encoding the human PLA2G1B （NP_000919.1） （Met 1-Ser 148） with a C-terminal polyhistidine tag was expressed.
組成：Lyophilized from sterile 10mM Tris, 5mM CaCl2, pH 8.0
保存条件：Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at ＜ -20℃ for 3 months.
