背景：TIGIT; also known as V-set and transmembrane domain-containing protein 3 （VSTM3） or V-set and immunoglobulin domain-containing protein 9 （VSIG9） is a new surface protein containing an immunoglobulin variable domain; a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif （ITIM）.
TIGIT is expressed on regulatory; memory; activated T cells and NK cells.
It binds PVR with high affinity; and PVRL2 with lower affinity; but not PVRL3.
Knockdown of TIGIT with siRNA in human memory T cells did not affect T cell responses; however; TIGIT inhibits NK cytotoxicity directly through its ITIM.
TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells.
The binding of PVR to TIGIT on human dendritic cells enhanced the production of IL-10 and diminished the production of IL-12p40.
In addition; TIGIT counter inhibits the NK-mediated killing of tumor cells and protects normal cells from NK-mediated cytotoxicity thus providing an "alternative self" mechanism for MHC class I inhibition.
仕様
サイズ：500ug
種由来：Human
発現宿主：HEK293 Cells
純度：＞85 ％ as determined by reducing SDS-PAGE.
エンドトキシン濃度：＜1.0 EU per μg of the protein as determined by the LAL method.
アクセッションNo.：Q495A1
アミノ酸配列：Met 1-Phe 138
遺伝子構造：A DNA sequence encoding the extracellular domain of human TIGIT （NP_776160.2） （Met 1-Phe 138） with a C-terminal polyhistidine tag was expressed.
別名（遺伝子名）：T-cell immunoreceptor with Ig and ITIM domains;;VSIG9; VSTM3;TIGIT;V-set and transmembrane domain-containing protein 3;V-set and immunoglobulin domain-containing protein 9
分子量：14.2 kDa
組成：Lyophilized from sterile PBS; pH 7.4
保存条件：Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at ＜ -20℃ for 3 months.
