背景：T-cell surface glycoprotein CD3 epsilon chain, also known as CD3E, is a single-pass type I membrane protein.
CD3E contains 1 Ig-like （immunoglobulin-like） domain and 1 ITAM domain.
CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex.
The CD3 epsilon subunit of the T cell receptor （TCR） complex contains two defined signaling domains, a proline-rich sequence and an immune tyrosine activation motifs （ITAMs）, and this complex undergoes a conformational change upon ligand binding that is thought to be important for the activation of T cells.
In the CD3 epsilon mutant mice, all stages of T cell development and activation that are TCR-dependent were impaired, but not eliminated, including activation of mature na＆iuml;ve T cells with the MHCII presented superantigen, staphylococcal enterotoxin B, or with a strong TCR cross-linking antibody specific for either TCR-Cbeta or CD3 epsilon.
T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors.
CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency.
Homozygous mutations in CD3D and CD3E genes lead to a complete block in T-cell development and thus to an early-onset severe combined immunodeficiency phenotype.
仕様
サイズ：500ug
種由来：Human
発現宿主：HEK293 Cells
純度：＞80 ％ as determined by reducing SDS-PAGE.
エンドトキシン濃度：＜1.0 EU per μg of the protein as determined by the LAL method.
アクセッションNo.：NP_000724.1
アミノ酸配列：Met 1-Asp 126
遺伝子構造：A DNA sequence encoding the extracellular domain （Met 1-Asp 126） of human CD3E （NP_000724.1） precursor was fused with a polyhistidine tag at the C-terminus.
保存条件：Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at ＜ -20℃ for 3 months.
