背景：Poly （ADP-ribose） polymerase 1（PRAP1）, also known as NAD（+） ADP-ribosyltransferase 1（ADPRT）, is a chromatin-associated enzyme which modifies various nuclear proteins by poly（ADP-ribosyl）ation.
The ADP-D-ribosyl group of NAD+ is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units.
The poly（ADP-ribosyl）ation modification is critical for a wide range of processes, including DNA repair, regulation of chromosome structure, transcriptional regulation, mitosis and apoptosis.
PARP1 is demonstrateed to mediate the poly（ADP-ribose） ation of APLF （aprataxin PNK-like factor） and CHFR （checkpoint protein with FHA and RING domains）, two representative proteins involved in the DNA damage response and checkpoint regulation.
Further, It has been suggested that DNA-dependent protein kinase （DNA-PK）, another component of DNA repair, suppresses PARP activity, probably through direct binding and/or sequestration of DNA-ends which serve as an important stimulator for both enzymes.
PARP1 inhibitors is thus proposed as a targeted cancer therapy for recombination deficient cancers, such as BRCA2 tumors.
仕様
サイズ：50ug
種由来：Human
発現宿主：Baculovirus-Insect Cells
純度：＞90 ％ as determined by reducing SDS-PAGE.
エンドトキシン濃度：＜1.0 EU per μg of the protein as determined by the LAL method.
アクセッションNo.：NP_001609.2
アミノ酸配列：Met 1-Trp 1014
遺伝子構造：The amino acids corresponding to the full length of human PARP1 （NP_001609.2） （Met 1-Trp 1014） was fused with a polyhistidine tag at the C-terminus.
保存条件：Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at ＜ -20℃ for 3 months.
