84-9362-77　Recombinant Human AGO2/Argonaute 2/EIF2C2 Protein （His Tag）（Active） 100ug　PKSH031271

特徴

• 背景：Argonaute 2 （AGO2）, also known as Eukaryotic translation initiation factor 2C2 （EIF2C2）, belongs to the Argonaute family, AGO subfamily, which is a component of the RNA-induced silencing complex （RISC） and mediates small interfering RNA （siRNA）-directed mRNA cleavage and microRNA translational suppression.
• AGO2 protein is the catalytic engine of mammalian RNAi.
• It contains a PIWI domain that is structurally related to RNases H and possibly shares with them a two-metal-ion catalysis mechanism.
• Human AGO2 was unable to cleave preformed RNA duplexes and exhibited weaker binding affinity for RNA duplexes compared with the single strand RNA.
• The enzyme exhibited greater RNase H activity in the presence of Mn2+ compared with Mg2+.
• Human AGO2 exhibited weaker binding affinities and reduced cleavage activities for antisense RNAs with either a 5'-terminal hydroxyl or abasic nucleotide.
• In mouse hematopoiesis, AGO2 controls early development of lymphoid and erythroid cells.
• AGO2 is a highly specialized member of the Argonaute family with an essential nonredundant Slicer-independent function within the mammalian miRNA pathway.
• AGO2 regulates dFMR1 expression, and the relationship between dFMR1 and AGO2 was defined by their physical interaction and co-regulation of downstream targets.
• AGO2 and dFMR1 are also connected through a regulatory relationship.
• AGO2 is a regulator of dFMR1 expression and have clarified an important developmental role for AGO2 in the nervous system and germ line that requires dFMR1 function.
• In addition, AGO2 is regulated at both the transcriptional and posttranslational level, and also implicate AGO2 and enhanced micro-RNA activity in the tumorigenic progression of breast cancer cell lines.