背景：INSR （Insulin receptor）, also known as CD220, is a transmembrane receptor that is activated by insulin.
INSR belongs to theprotein kinase superfamily, and exists as a tetramer consisting of two alpha subunits and two beta subunits linked by disulfide bonds.
The alpha and beta subunits are encoded by a single INSR gene, and the beta subunits pass through the cellular membrane.
As the receptor for insulin with tyrosine-protein kinase activity, INSR associates with downstream mediators upon binding to insulin, including IRS1 （insulin receptor substrate 1） and phosphatidylinositol 3'-kinase （PI3K）.
IRS-1 binding and phosphorylation eventually leads to an increase in the high affinity glucose transporter （Glut4） molecules on the outer membrane of insulin-responsive tissues.
INSR isoform long and isoform short are expressed in the peripheral nerve, kidney, liver, striated muscle, fibroblasts and skin, and is found as a hybrid receptor with IGF1R which also binds IGF1 in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibrobasts, spleen and placenta.
Defects in Insulin Receptor/INSR are the cause of Rabson-Mendenhall syndrome （Mendenhall syndrome）, insulin resistance （Ins resistance）, leprechaunism （Donohue syndrome）, and familial hyperinsulinemic hypoglycemia 5 （HHF5）.
It may also be associated with noninsulin-dependent diabetes mellitus （NIDDM）.
仕様
サイズ：1mg
種由来：Human
発現宿主：HEK293 Cells
純度：＞95 ％ as determined by reducing SDS-PAGE.
エンドトキシン濃度：＜1.0 EU per μg of the protein as determined by the LAL method.
アクセッションNo.：NP_000199.2
アミノ酸配列：Met 1-Lys 956
遺伝子構造：A DNA sequence encoding the human INSR isoform long （NP_000199.2） extracellular domain （Met 1-Lys 956） was expressed, fused with a polyhistidine tag at the C-terminus.
別名（遺伝子名）：CD220;HHF5;Insulin Receptor
分子量：107 （83+24） kDa
組成：Lyophilized from sterile PBS, pH 7.4
保存条件：Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at ＜ -20℃ for 3 months.
