背景：Ectonucleoside triphosphate diphosphohydrolase 5 （ENTPD5）, also known as CD39 antigen-like 4, ER-UDPase, Guanosine-diphosphatase ENTPD5, Nucleoside diphosphatase Uridine-diphosphatase ENTPD5.
This hydrolase is expressed in response to phosphoinositide 3-kinase （PI3K） signaling.
Activation of PI3K results in FOXO phosphorylation by AKT1 and loss of ENTPD5 transcriptional repression.
It is Up-regulated in PTEN-deficient cells.
Uridine diphosphatase （UDPase） that promotes protein N-glycosylation and ATP level regulation.ENTPD5 promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle.
Together with CMPK1 and AK1, ENTPD5 constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis.
ENTPD5 also hydrolyzes GDP and IDP but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate.
This enzyme Plays a key role in the AKT1-PTEN signaling pathway by promoting glycolysis in proliferating cells in response to phosphoinositide 3-kinase （PI3K） signaling.
仕様
サイズ：50ug
種由来：Human
発現宿主：Baculovirus-Insect Cells
純度：＞90 ％ as determined by reducing SDS-PAGE.
エンドトキシン濃度：＜1.0 EU per μg of the protein as determined by the LAL method.
アクセッションNo.：O75356
アミノ酸配列：Met 1-His 428
遺伝子構造：A DNA sequence encoding the human ENTPD5 （O75356） （Met 1-His 428） was fused with a polyhistidine tag at the C-terminus.
保存条件：Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at ＜ -20℃ for 3 months.
