背景:Biotinidase, also known as biotinase and BTD, is a ubiquitous mammalian cell enzyme which expressed at high levels in the liver, serum, and kidney.
Its primary function is to cleave biotin from biocytin, preserving the pool of biotin for use as a cofactor for biotin dependent enzymes, namely the 4 human carboxylases.
Biotinidase also recycles biotin from enzymes in the body that use it as a helper component in order to function.
These enzymes, known ascarboxylases, are important in the processing offats,carbohydrates, andproteins.
Biotin is attached to these carboxylase enzymes through anamino acid(the building material of proteins) calledlysine, forming a complex calledbiocytin.
仕様
サイズ:50ug
種由来:Human
発現宿主:HEK293 Cells
純度:>95 % as determined by reducing SDS-PAGE.
エンドトキシン濃度:<1.0 EU per μg of the protein as determined by the LAL method.
アクセッションNo.:P43251
アミノ酸配列:Met 1-Asp543
遺伝子構造:A DNA sequence encoding the human BTD (P43251) (Met1-Asp543) with a C-terminal polyhistidine tag was expressed.
別名(遺伝子名):BTD
分子量:58.2 kDa
組成:Lyophilized from sterile PBS, pH 7.4
保存条件:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.