背景：Interleukin 23 （IL-23） is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12.
The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11.
The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors.
Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23.
IL-23 has biological activities that are similar to, but distinct from IL-12.
Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells.
While IL-12 acts on both naive and memory human T cells, the effects of IL-23 is restricted to memory T cells.
仕様
サイズ：100ug
種由来：Human
発現宿主：HEK293 Cells
純度：＞90 ％ as determined by reducing SDS-PAGE.
エンドトキシン濃度：＜1.0 EU per μg of the protein as determined by the LAL method.
アクセッションNo.：Q9NPF7＆NP_002178.2
アミノ酸配列：Met 1-Pro189＆Met 1-Ser 28
遺伝子構造：A DNA sequence encoding the IL23A （Q9NPF7） （Met 1-Pro 189） was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the p40 subunit of human IL12, termed as IL12B （NP_002178.2） （Met 1-Ser 328） was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the IL23 heterodimer was purified.
保存条件：Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at ＜ -20℃ for 3 months.
