背景：The Fc receptor with low affinity for IgG （FCGR3, or CD16） is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms.
FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer （NK） cells, and a subset of T cells.CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity （ADCC） by binding to the Fc portion of various antibodies.
CD16a / FCGR3A also has a broader function.
CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding.
Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor.
CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation.
仕様
サイズ：100ug
種由来：Human
発現宿主：HEK293 Cells
純度：＞95 ％ as determined by reducing SDS-PAGE.
エンドトキシン濃度：＜1.0 EU per μg of the protein as determined by the LAL method.
アクセッションNo.：P08637-1
アミノ酸配列：Met 1-Gln 208
遺伝子構造：A DNA sequence encoding the extracellular domain （Met1-Gln208） of human CD16a （P08637-1, with natural variant Phe 176 Val） was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus （AVI-his）. The expressed protein was biotinylated in vivo by the Biotin–Protein ligase （BirA enzyme） which is co-expressed.
別名（遺伝子名）：Low Affinity Immunoglobulin Gamma Fc Region Receptor III-A; CD16a Antigen; Fc-Gamma RIII-Alpha; Fc-Gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa; FcR-10; IgG Fc Receptor III-2; CD16a; FCGR3A; CD16A; FCG3; FCGR3; IGFR3;CD16;CD16A
分子量：25.6 kDa
組成：Lyophilized from sterile PBS, pH 7.4
保存条件：Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at ＜ -20℃ for 3 months.
