84-9354-15　［取扱停止］Recombinant E.coli Glycerol kinase Protein （His Tag） 100ug　PDEO100001

特徴

• 背景：Glycerol kinase fromE.
• coli（glpK） catalyzes the ATP-dependent phosphorylation of glycerol to producesn-glycerol-3-phosphate （G3P）, the first and rate-limiting step in the utilization of glycerol.
• In the presence of glycerol, glpK is stimulated by interaction with the membrane-bound glycerol facilitator.
• In the presence of glucose, glpK activity is allosterically inhibited by fructose-1,6-bisphosphate （FBP） of the glycolytic pathway.
• Under physiological conditions, the enzyme is in an equilibrium between the active dimer and the inactive tetramer.
• FBP binds to and stabilizes the inactive form, therefore shifting the usage of glycerol metabolic pathway to glycolytic pathway.
• GlpK is a member of a superfamily of ATPases that includes actin, hexokinase and the heat shock protein hsc70.
• Although these proteins are dissimilar in amino acid sequence and function, they share similar tertiary folds and likely the same catalytic mechanism.
• The enzyme activity was measured using a phosphatase-coupled kinase assay.