84-9345-13　［取扱停止］Recombinant Human RAC1 protein（His tag） 20ug　PDEH100097

特徴

• 背景：RAC1 is a GTPase that belongs to the RAS superfamily of small GTP-binding proteins.
• Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases.
• Two transcript variants encoding different isoforms have been found for RAC1 gene.
• RAC1 is a plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states.
• In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.
• RAC1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophage.
• RAC1 is essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly.
• RAC1's isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins.
• It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction.
• Stat3 is an important transcription factor that regulates both proinflammatory and anti-apoptotic pathways in the heart.
• It forms a multiprotein complex with RAC1 and PKC in an H/R-dependent manner by expression of constitutively active Rac1 mutant protein, and by RNA silencing of RAC1.
• Selective inhibition of PKC with calphostin C produces a marked suppression of Stat3 S727 phosphorylation.
• The association of Stat3 with Rax1 occurs predominantly at the cell membrane, but also inside the nucleus, and occurs through the binding of the coiled-coil domain of Stat3 to the 54 NH（2）-terminal residues of RAC1.
• Transfection with a peptide comprising the NH（2）-terminal 17 amino acid residues of RAC1-dependent signaling pathways resulting in a physical association between Rac1 and Stat3 and the formation of a novel multiprotein complex with PKC.