HGF is a potent, mesenchymally-derived mitogen for mature parenchymal hepatocytes, and acts as a growth factor for a broad spectrum of tissues and cell types.
HGF signals through a transmembrane tyrosine kinase receptor known as MET.
Activities of HGF include the induction of cell proliferation, motility, morphogenesis, inhibition of cell growth, and enhancement of neuron survival.
HGF is a crucial mitogen for liver regeneration processes, especially after partial hepatectomy and other liver injuries.
Human and murine HGF are cross-reactive.
Human HGF is expressed as a linear, polypeptide-precursor glycoprotein containing 697 amino acid residues.
Proteolytic processing of this precursor generates the biologically active heterodimeric form of HGF, which consists of two polypeptide chains （＆alpha;-chain and β-chain） held together by a single disulfide bond resulting in formation of a biologically active heterodimer.
The ＆alpha;-chain consists of 463 amino acid residues and four kringle domains.
The β-chain consists of 234 amino acid residues.
The BioGems Human HGF Pre-Coated ELISA （Enzyme-Linked Immunosorbent Assay） kit is a solid phase immunoassay specially designed to measure Human HGF with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for HGF.
It contains sf21-expressed recombinant Human HGF.
The detection antibody is a biotinylated goat polyclonal antibody specific for HGF.
The kit is analytically validated with ready to use reagents.
