CD40, a member of the TNF receptor family, is a cell surface protein expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells.
Signaling though CD40 plays an important role in the proliferation and differentiation of B cells, and is critical for immunoglobulin （Ig） class switching.
The membrane-anchored CD40-Ligand is expressed almost exclusively on activated CD4+ T lymphocytes.
Failure to express CD40L leads to "immunodeficiency with hyper-IgM", a disease characterized by failure to produce IgG, IgA and IgE.
The human CD40L gene codes for a 261 amino acid type II transmembrane protein, which contains a 22 amino acid cytoplasmic domain, a 24 amino acid transmembrane domain, and a 215 amino acid extracellular domain.
The BioGems Human CD40 Ligand Pre-Coated ELISA （Enzyme-Linked Immunosorbent Assay） kit is a solid phase immunoassay specially designed to measure CD40 Ligand with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for CD40 Ligand.
It contains E.coli-expressed recombinant CD40 Ligand.
The detection antibody is a biotinylated goat polyclonal antibody specific for CD40 Ligand.
The kit is analytically validated with ready to use reagents.
