ANG-2 binds to the endothelial cell specific receptor Tie-2, but, in contrast to ANG-1, does not induce tyrosine phosphorylation.
Consequently, ANG-2 modulates ANG-1 activation of Tie-2 and, depending on the physiological and biochemical environment, can act either as an agonist or antagonist of Tie-2 induced angiogenesis.
The signaling interactions of ANG-1, ANG-2 and Tie-2, along with less characterized ANG-3 and ANG-4, are required for embryonic and adult angiogenesis.
Physiologically, ANG-1 and ANG-2 are associated with sprouting, tube formation, and structural integrity of newly formed blood vessels.
Mature human ANG-2 is a secreted protein containing 480 amino acid residues.
ANG-2 is composed of an alpha-helix-rich "coiled coil" N-terminal domain and fibrinogen-like C-terminal domain.
ANG-2 exists predominantly in the form of a disulfide-linked dimer.
The BioGems Human ANG-2 Pre-Coated ELISA （Enzyme-Linked Immunosorbent Assay） kit is a solid phase immunoassay specially designed to measure ANG-2 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for ANG-2.
It contains NS0-expressed recombinant ANG-2.
The detection antibody is a biotinylated goat polyclonal antibody specific for ANG-2.
The kit is analytically validated with ready to use reagents.
