The 16-10A1 antibody reacts with mouse CD80, also known as B7-1, a 55 kDa type I transmembrane protein ligand for CD152 （CTLA-4） and for CD28, a co-stimulatory receptor for the T cell receptor （TCR）.
CD28 also binds a second B7 ligand known as CD86 （B7-2）.
Both CD80 and CD86 are expressed on activated B cells and antigen-presenting cells.
These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity.
The interaction / co-stimulatory signaling between the B7 ligands and CD28 or CTLA-4 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response.
