背景：Rabbit pulmonary artery fibroblasts are isolated from pulmonary artery tissue. The pulmonary aorta is also called the pulmonary trunk. In air-breathing vertebrates, the arteries that direct venous blood from the heart to the lungs. The aorta pulmonary artery originates from the right ventricle and runs diagonally to the upper left and rear before the aorta. It is divided into the left and right pulmonary arteries under the aortic arch and enters the lungs through the hilum. The trunk of the pulmonary artery is a stubby trunk located in the pericardium. It starts from the right ventricle and runs diagonally from the front of the ascending aorta to the upper left, back and upper, to the left and right pulmonary arteries below the aortic arch. The left pulmonary artery is short, running across the front of the left main bronchus, dividing into two branches into the upper and lower lobes of the left lung. The right pulmonary artery is long and thick. It runs transversely to the right after passing through the ascending aorta and superior vena cava, and divides into three branches into the upper, middle and lower lobes of the right lung at the right hilum. Fibroblasts are the main cellular components of loose connective tissue, which are derived from the mesenchyme in the embryonic period. Fibroblasts are large, with clear outlines, mostly protruding spindle-shaped or star-shaped flat structures, with regular oval nuclei and large and obvious nucleoli. Fibroblasts have vigorous functions and activities. The cytoplasm is weakly basophilic and has obvious protein synthesis and secretion activities. Under certain conditions, it can achieve mutual transformation with fibroblasts. Fibroblasts are very important for the repair of different degrees of cell degeneration, necrosis and tissue defects Effect. The newly isolated pulmonary artery fibroblasts are round and have good refractive index. They are suspended in the culture medium. After 30 minutes, the cells adhere to the flask, and some of them begin to protrude pseudopods, which appear as small protrusions. After 6 hours, the cells are almost completely attached. It stretches into a fusiform with clear nuclei, relatively uniform distribution, scattered growth, and does not aggregate into clusters. The cells grow rapidly and reach confluence within 5-7 days. The cells are tightly arranged, and some overlap and grow, flat and large. The cytoplasm is transparent, the nucleus is large, elliptical, and light in color. The cells reach confluence and connect to each other into a network. The cells are distributed in a protruding spindle or star-shaped flat. The main functions of lung fibroblasts under physiological conditions include： constructs and maintains the normal form of lung organs, synthesizes and releases extracellular matrix, and collects and repairs damaged tissues in a timely manner after tissue damage.The rabbit lung fibroblasts produced by our company are prepared by trypsin-collagenase mixed digestion method combined with differential adhesion method, the total number of cells is about 5×10^5 cells/vial. The cells are identified by Vimentin immunofluorescence, and the purity is more than 90％ without HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi, etc.
