LOX-1 is a type II transmembrane protein belonging to the C-type lectin family.
It is highly expressed on endothelial cells and functions as an endocytosis receptor for atherogenic oxidized LDL.
Soluble LOX-1 can be generated from the transmembrane protein by proteolytic cleavage.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA (detection): 0.1-0.4ug/ml with L2623-01C as capture antibodyWestern Blot: 0.1ug/ml using recombinant mouse LOX‑1/OLR1Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, BSA. Labeled with Biotin. Reconstitute with 250ul sterile PBS.
Specificity：Recognizes mouse LOX-1 at C-terminal. Less than 0.2% cross-reactivity with recombinant human (rh) LOX-1 and human LDL and ~5% cross-reactivity with rhLOX-1.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Arg60-Ile363 from mouse LOX-1, expressed in NS0 cells.
