84-6253-93　Mycobacterium tuberculosis ESAT6 100ug　M9699-58J

特徴

• Mycobacterium tuberculosis is the most common cause of tuberculosis.
• Primary infection begins with inhalation of 1 to 10 aerosolised bacilli.
• The pathogenicity of the organism is determined by its ability to escape host immune responses as well as eliciting delayed hypersensitivity.
• Alveolar macrophages engulf the invading cells but are unable to mount an effective defense.
• Several virulence factors are responsible for this apparent failure; most notably in the mycobacterial cell wall are the cord factor, lipoarabinomannan, and the 65kD heat shock protein or HSP65.
• The emergence of new strains of resistant Mycobacterium tuberculosis has created new interest in clinical diagnosis.
• Studies have shown immunohistochemical techniques to be superior to conventional special stains.
• Thus the demonstration of mycobacterial antigens are not only useful in establishing mycobacterial aetiology, but can also be used as an alternative method to the conventional Ziehl-Neelsen method.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilutions:Western blotting analysis (reducing conditions) of recombinant protein ESAT6 using ESAT6 polyclonal antibody
• Lane 1,3, 5: Cell lysates of non - transfected E.
• coli (negative controls).
• Lane 2,4,6 : Cell lysate of ESAT6 - transfected E.
• coli; dilution (2) 1ug/ml, (4) 0.2ug/ml , (6) 0.1ug/ml; detection with Donkey anti-rabbit/HRP secondary antibody
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.