84-6253-72　TIM-3 100ug　583303

特徴

• The immune checkpoint protein TIM-3 is a member of the immunoglobulin superfamily and TIM family of proteins that was initially identified as a specific marker of fully differentiated IFN-gamma producing CD4 T helper 1 (Th1) and CD8 cytotoxic cells.
• It is a Th1-specific cell surface protein that regulates macrophage activation and negatively regulates Th1-mediated auto- and alloimmune responses, and is also highly expressed on regulatory T cells, monocytes, macrophages, and dendritic cells (1).
• TIM-3 and PD-1 are co-expressed on most CD4 and CD8 T cells infiltrating solid tumors or in hematologic malignancy in mice; blocking TIM-3 in conjugation with a PD-1 blockade increases the functionality of exhausted T cells and synergizes with to inhibit tumor growth (2,3).
• Applications:Suitable for use in ELISA, Immunofluorescence, Immunohistochemistry and Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-2ug/mlImmunohistochemistry (paraffin): 2ug/mlImmunofluorescence: 20ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control:Jurkat Cell Lysate
• Molecular Weight:~33kD (predicted), 37kD (observed)
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.