STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors.
After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes.
Among the seven STATs (STAT1, STAT2, STAT3, STAT4, STAT5a, STAT5b, and STAT6), STAT1, STAT3, STAT5a, and STAT5b have a wide activation profile.
STAT3 signals are pivotal to the proliferation and differentiation of neural stem cells and also participate in neuronal regeneration and cancers of the nervous system.
Applications:Suitable for use in ELISA, Immunofluorescence, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/mlImmunohistochemistry: paraffinOptimal dilutions to be determined by the researcher.
Positive Control:Human Small Intestine Tissue Lysate, Human Small Intestine Tissue Slide
Molecular Weight:~85kD (predicted)
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human STAT3. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to 19 amino acids, located within amino acids 170-220, near the N-terminus of human STAT3. Species sequence homology: bovine: (100%), porcine: (100%) and chicken: (100%)
