The polymerase I and transcript release factor (PTRF) enables the dissociation of paused ternary polymerase I transcription complexes from the 3' end of pre-rRNA transcripts, regulating rRNA transcription by promoting the dissociation of transcription complexes and the re-initiation of polymerase I on nascent rRNA transcripts.
PTRF also localizes to caveolae at the plasma membrane and is thought to play a critical role in the formation of caveolae and the stabilization of caveolins, translocating from caveolae to the cytoplasm after insulin stimulation.
PTRF is also thought to modify lipid metabolism and insulin-regulated gene expression.
Mutations in this gene result in a disorder characterized by generalized lipodystrophy and muscular dystrophy.
Applications:Suitable for use in Immunohistochemistry, Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Human Spleen Tissue Lysate, Human Spleen Tissue Slide
Molecular Weight:~43kD
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human PTRF.
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 19 amino acid near the C-terminus of human PTRF. Species sequence homology: rat: (95%) and mouse: (95%)
