PIERCE1, also known as C9orf116, is an important p53 target gene contributing to normal DNA damage response and may play crucial roles in maintaining genomic integrity against genotoxic stresses, including UVC irradiation.
PIERCE1 exists as two alternatively spliced isoforms and is predominantly expressed in human small cell lung cancer.
PIERCE1 knockdown induces down-regulation of proapoptotic genes including Ei24, Apaf1 and PTEN, suggesting that PIERCE1 is a proapoptotic gene.
It is helpful for in-depth understanding of p53 pathways and the design of new cancer therapies.
Applications:Suitable for use in ELISA, Immunocytochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlImmunocytochemistry: 2.5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:A20 Cell Lysate, A-20 Cell Slide
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human PIERCE1 Predicted to not cross-react with other UPF0691 family members.. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to 16 amino acids near the N-terminus of human PIERCE1.
