PHLPP2 is a member of the serine/threonine phosphatase family, which are important regulators of Akt serine-threonine kinases (AKT1, AKT2, AKT3) and conventional/novel protein kinase C (PKC) isoforms.
PHLPP1 and PHLPP2 have a similar domain structure and have been shown to dephosphorylate and inactivate, distinct Akt isoforms, at one of the two critical phosphorylation sites required for activation: Serine473.
PHLPP2 dephosphorylates AKT1 and AKT3, whereas PHLPP1 is specific for AKT2 and AKT3.
PHLPP1 promotes apoptosis and may act as a tumor suppressor.
PHLPP2 associates with and is inhibited by adenylyl cyclase type 6 (AC6), thereby allowing Akt activation.
Applications:Suitable for use in ELISA, Immunofluorescence, Immunocytochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:SW480 Cell Lysate
Molecular Weight:~156kD
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human PHLPP2.
Isotype：IgG
Calc Applications Abbrev：E IC IF WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 20 amino acids near the C-terminus of human PHLPP2.
