Kremen (Kringle containing protein marking the eye and the nose) proteins are type I transmembrane proteins that contain extracellular kringle, WSC and CUB domains and an intracellular region without any conserved motifs.
Kremens bind a subset of the secreted Dickkopf proteins (Dkk 1, 2, and 4) with high affinity to modulate the canonical Wnt signaling pathway that is transduced by the ternary receptor complex composed of Wnt, Frizzled, and the LDL receptor related protein 5/6 (LRP5/6) coreceptor.
KREMEN1 is a receptor for the Dickkopf protein which blocks Wnt/beta catenin signaling.
It is necessary to ensure normal spatial and temporal patterns of Wnt activity during developmental processes.
Applications:Suitable for use in ELISA, Immunohistochemistry, Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.125-0.25ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Rat Small Intestine Tissue Lysate, Human Small Intestine Tissue Slide
Molecular Weight:~54kD
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human KREMEN1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to 18 amino acid near the C-terminus of human KREMEN1. Species sequence homology: rat: (94%) and mouse: (94%)
