FOXP3 is a member of the forkhead/winged-helix family of transcriptional regulators.
FOXP3 acts as a repressor of transcription and regulates T cell activation, with its overexpression in CD4 T cells leading to an attenuation of activation-induced cytokine production and proliferation.
In regulatory T (Treg) cells, FOXP3 is essential for Treg suppressor function and its expression leads to the repression of IL-17 expression.
Genetic mutations involving FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome.
Applications:Suitable for use in ELISA, Immunofluorescence, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/mlImmunohistochemistry: paraffinOptimal dilutions to be determined by the researcher.
Positive Control:A549 Cell Lysate
Molecular Weight:~47kD (predicted)
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human FOXP3. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to 15 amino acid near the C-terminus of human FOXP3.
