DISP1 is the mammalian homolog of the Drosophila Dispatched segment-polarity gene and a key regulator of the Hedgehog (Hh) signaling pathway during embryonic development.
DISP1 is required to move Shh from site of synthesis during embryogenesis; mutations in the gene result in lethality at mid-gestation and prevents specification of ventral cell types in the neural tube.
Recent results have shown that DISP1 mediates the basolateral secretion of Shh and regulates growth of mammalian long bones through the control of Ihh.
Applications:Suitable for use in ELISA, Immunohistochemistry, Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/mlImmunohistochemistry (paraffin): 2.5ug/mlImmunofluorescence: 20ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:3T3 Cell Lysate, Human Kidney Tissue Slide
Molecular Weight:~29kD
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human DISP1. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to 16 amino acids, aa20-70, near the N-terminus of human DISP1.
