84-6252-75　CDKN2A [KO Validated] 100ug　583167

特徴

• The CDKN2A locus gives rise to 2 distinct transcripts from different promoters.
• The transcripts have been designated p16(INK4A) and p14(ARF).
• This chromosomal region undergoes a number of inversions, translocations, heterozygous deletions, and homozygous deletions in a variety of malignant cell lines including those from glioma, non-small cell lung cancer, leukemia, and melanoma.
• Deletion of the region containing CDKN2A is found in more than half of all melanoma cell lines.
• Conversely, transfection of CDKN2A suppressed the growth of two independent mesothelioma cell lines, suggesting that inactivation of the CDKN2 gene is an essential step in the etiology of malignant mesotheliomas.
• CDKN2A induces a G1 cell cycle arrest by inhibiting the phosphorylation of the Rb protein by the cyclin-dependent kinases CDK4 and CDK6.
• CDKN2A is expressed as at least three distinct isoforms.
• Applications:Suitable for use in ELISA, Immunohistochemistry and Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-2ug/mlImmunohistochemistry (paraffin): 5ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control:HeLa Cell Lysate, HepG2 Cell Lysate
• Molecular Weight:~17kD (predicted), 17kD (observed)
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.