84-6252-73　CD80 100ug　583159

特徴

• CD80, also known as B7-1, is a type I membrane protein that is a member of the immunoglobulin superfamily.
• Like the related protein CD86, this protein is expressed by antigen-presenting cells, and is the ligand for two proteins at the cell surface of T cells, CD28 and the cytotoxic T-lymphocyte-associated protein 4 (CTLA-4).
• Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell and induces T-cell proliferation and cytokine production.
• CTLA-4 binding negatively regulates T-cell activation and diminishes the immune response (1).
• Blocking the CTLA-4-CD80/CD86 interaction has been shown to enhance T-cell functions in acute lymphoblastomic leukemia (ALL), suggesting that this pathway may be an attractive target for future cancer immunotherapy (2).
• Applications:Suitable for use in ELISA, Immunohistochemistry, Immunofluorescence and Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-2ug/mlImmunohistochemistry (paraffin): 2ug/mlImmunofluorescence: 20ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control:EL4 Cell Lysate
• Molecular Weight:~120kD (predicted), 120kD (observed)
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.