Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3').
Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206.
Critical for early embryogenesis and for embryonic stem cell pluripotency.
Applications:Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:2000Immunohistochemistry (paraffin): 1:25Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity：Recognizes mouse Pou5f1.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Mo
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within aa86-119 from mouse Pou5f1.
