Tyrosine kinase that plays an essential role in regulation of the adaptive immune response.
Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development.
Contributes also to the development and activation of primary B-lymphocytes.
When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD3Z through ITAM motif at the plasma membrane.
This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation.
Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK.
Subsequently, ZAP70 phosphorylates at least 2 essential adapter proteins.
Applications:Suitable for use in FLISA.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes mouse Zap70.
Isotype：IgG
Calc Applications Abbrev：FLISA
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to aa253-32 from mouse tyrosine-protein kinase ZAP-70.
