Tyrosine kinase that plays an essential role in regulation of the adaptive immune response.
Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development.
Contributes also to the development and activation of primary B-lymphocytes.
When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane.
This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation.
Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK.
Subsequently, ZAP70 phosphorylates at least 2 essential adapter proteins
Applications:Suitable for use in Immunohistochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:50-300ELISA: 1:10,000-20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human ZAP70.
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa391-440 from human ZAP70.
