Hydrolase that can remove conjugated ubiquitin from proteins and participates in endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins.
May act by triming the ubiquitin chain on the associated substrate to facilitate their threading through the VCP/p97 pore.
Ubiquitin moieties on substrates may present a steric impediment to the threading process when the substrate is transferred to the VCP pore and threaded through VCP's axial channel.
Mediates deubiquitination of 'Lys-27'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitin chains.
Also able to hydrolyze 'Lys-11'-linked ubiquitin chains.
Cleaves both polyubiquitin and di-ubiquitin.
May play a role in macroautophagy, regulating for instance the clearance of damaged lysosomes.
May recruit PLAA, UBXN6 and VCP to damaged lysosome membranes decorated with K48-linked ubiquitin chains and remove these chains allowing autophagosome formation
Applications:Suitable for use in ELISA and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Specificity：Recognizes human YOD1.
Isotype：IgG
Calc Applications Abbrev：E IF
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa1-44 from human ubiquitin thioesterase OTU1 protein.
