Seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family.
Inhibits anti-caspase activity of BIRC4.
Induces cleavage and inactivation of BIRC4 independent of caspase activation.
Mediates TNF-alpha-induced apoptosis and is involved in apoptosis in trophoblast cells.
May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway.
After translocation to mitochondria, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria.
Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm.
The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4.
Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL.
May be a tumor suppressor by mediating apoptosis resistance of cancer cells.
Applications:Suitable for use in ELISA, Immunohistochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:20-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Specificity：Recognizes human XAF1.
Isotype：IgG
Calc Applications Abbrev：E IF IHC
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa172-259 from human XIAP-associated factor 1 protein.
