Ligand for members of the frizzled family of seven transmembrane receptors.
Can activate or inhibit canonical Wnt signaling, depending on receptor context.
In the presence of FZD4, activates beta-catenin signaling.
In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves downregulation of beta-catenin-induced reporter gene expression.
Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation.
Stimulates cell migration.
Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor.
Mediates motility of melanoma cells.
Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle.
Inhibits type II collagen expression in chondrocytes
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:5000Immunohistochemistry: 1:20-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Specificity：Recognizes human WNT5A. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding to aa62-380 from human protein Wnt-5a protein.
