Mediates the side-chain deamidation of N-terminal glutamine residues toglutamate, an important step in N-end rule pathway of protein degradation
Conversion of the resulting N-terminal glutamine to glutamate renders theprotein susceptible to arginylation, polyubiquitination and degradation asspecified by the N-end rule.
Does not act on substrates with internal or Cterminal glutamine and does not act on non-glutamine residues in any position.
Does not deaminate acetylated N-terminal glutamine.
With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity.
In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine (By similarity).
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Specificity：Recognizes human WDYHV1. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding to aa1-205 from human protein N-terminal glutamine amidohydrolase protein.
