E3 ubiquitin-protein ligase which is a component of the N-end rule pathway (By similarity).
Does not bind to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation (By similarity).
May play a role in Shh signaling by mediating the ubiquitination of Kif7 (By similarity).
May be important for MYH9 function in certain tissues, possibly by regulating the ubiquitination of MYH9 and consequently affecting its interaction with MYO7A.
Applications:Suitable for use in ELISA, Immunohistochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:500-1:1000Immunofluorescence: 1:200-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Specificity：Recognizes human UBR3.
Isotype：IgG
Calc Applications Abbrev：E IF IHC
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa960-1113 from human E3 ubiquitin-protein ligase UBR3 protein.
