Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities.
Involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity.
Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways.
In cooperation with TAX1BP1 promotes disassembly of E2-E3 ubiquitin protein ligase complexes in IL-1R and TNFR-1 pathways; affected are at least E3 ligases TRAF6, TRAF2 and BIRC2, and E2 ubiquitin-conjugating enzymes UBE2N and UBE2D3.
In cooperation with TAX1BP1 promotes ubiquitination of UBE2N and proteasomal degradation of UBE2N and UBE2D3.
Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains.
This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B.
Deubiquitinates TRAF6 probably acting on 'Lys-63'-linked polyubiquitin.
Upon T-cell receptor (TCR)-mediated T-cell activation, deubiquitinates 'Lys-63'-polyubiquitin chains on MALT1 thereby mediating disassociation of the CBM (CARD11:BCL10:MALT1) and IKKcomplexes and preventing sustained IKK activation.
DeubiquitinatesNEMO/IKBKG; the function is facilitated by TNIP1 and leads to inhibition of NFkappa-B activation.
Upon stimulation by bacterial peptidoglycans, probably deubiquitinates RIPK2.
Can also inhibit I-kappa-B-kinase (IKK) through a noncatalytic mechanism which involves polyubiquitin; polyubiquitin promotes association with IKBKG and prevents IKK MAP3K7-mediated phosphorylation.
Targets TRAF2 for lysosomal degradation.
In vitro able to deubiquitinate 'Lys-11'-, 'Lys-48'- and 'Lys-63' polyubiquitin chains.
Inhibitor of programmed cell death.
Has a role in the function of the lymphoid system.
Required for LPSinduced production of proinflammatory cytokines and IFN beta in LPS-tolerizedmacrophages.
Applications:Suitable for use in ELISA, Western Blot and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Western Blot: 1:2000-1:10,000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Specificity：Recognizes human TNFAIP3. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IF WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding to aa97-183 from human tumor necrosis factor alpha-induced protein 3 protein.
