General transcription factor that functions at the core of the DNA-bindingmultiprotein factor TFIID.
Binding of TFIID to the TATA box is the initialtranscriptional step of the pre-initiation complex (PIC), playing a role in theactivation of eukaryotic genes transcribed by RNA polymerase II.
Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependenttranscription.
The rate of PIC formation probably is primarily dependent on therate of association of SL1 with the rDNA promoter.
SL1 is involved instabilization of nucleolar transcription factor 1/UBTF on rDNA.
Applications:Suitable for use in ELISA, Immunohistochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:20-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Specificity：Recognizes human TBP.
Isotype：IgG
Calc Applications Abbrev：E IF IHC
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa1-55 from human TATA-box-binding protein.
