SOCS family proteins form part of a classical negative feedback system thatregulates cytokine signal transduction.
SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway.
Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors.
Binding to JAK2 inhibits its kinase activity.
Suppresses fetal liver erythropoiesis.
Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells.
Regulates IL-6 signaling in vivo.
Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligasecomplex which mediates the ubiquitination and subsequent proteasomaldegradation of target proteins.
Seems to recognize IL6ST.
Applications:Suitable for use in ELISA, Immunohistochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:20-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Specificity：Recognizes human SOCS3.
Isotype：IgG
Calc Applications Abbrev：E IF IHC
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa1-224 from human suppressor of cytokine signaling 3 protein.
